Botony 5th Sem Notes Free PDF Download Kashmir University Study Material JK Crown

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- Define Microscopy & its types.

Ans: -Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye. There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy.
Explain the structure and principle used in light microscope.
Ans: -The compound microscope which is most commonly used today contains several lenses that magnify the image of a specimen under study. The total magnification of the object is a product of the magnification of the individual lenses; if the objective lens magnifies 100 -fold (a 100x lens, usually employed) and the eye piece magnifies 10- fold, the final magnification recorded by the human eye or on film will be 1000- fold (100 x 10). The limit of resolution of a light microscope using visible light is about 0.2μ μm (200nm). No matter how many times the image is magnified, the microscope



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